Spontaneous Fermentation in Sourdough An Ancestral Alliance Between Microorganisms and Breadmaking
DOI:
https://doi.org/10.70221/rgc.v4i2.89Keywords:
Spontaneous fermentation, Sourdough, Lactic acid bacteria, FODMAPs, PhytatesAbstract
Sourdough is an ancestral fermentative biotechnology based on the symbiosis between lactic acid bacteria and native yeasts, capable of transforming flour and water into bread with remarkable sensory and nutritional properties. Unlike industrial cultures, this spontaneous fermentation creates a unique microbial ecosystem that varies according to environmental conditions, flour type, hydration, and temperature.
During the process, acids, alcohols, and esters are produced, contributing to flavor and aroma; phytic acid is degraded, enhancing mineral absorption; and FODMAPs are reduced, improving digestibility. Additionally, specific strains such as Lpb. plantarum G8 produce natural antifungal compounds that extend the shelf life of bread without preservatives. This review bridges science and tradition, positioning sourdough as a contemporary tool for healthier and more sustainable baking.
Downloads
References
Akamine, I. T., Mansoldo, F. R. P., & Vermelho, A. B. (2023). Probiotics in the Sourdough Bread Fermentation: Current Status. Fermentation, 9(2). https://doi.org/10.3390/fermentation9020090 DOI: https://doi.org/10.3390/fermentation9020090
Calvert, M. D., Madden, A. A., Nichols, L. M., Haddad, N. M., Lahne, J., Dunn, R. R., & McKenney, E. A. (2021). A review of sourdough starters: ecology, practices, and sensory quality with applications for baking and recommendations for future research. PeerJ, 9, 1–37. https://doi.org/10.7717/peerj.11389 DOI: https://doi.org/10.7717/peerj.11389
Cristofori, F., Francavilla, R., Capobianco, D., Dargenio, V. N., Filardo, S., & Mastromarino, P. (2020). Bacterial-Based Strategies to Hydrolyze Gluten Peptides and Protect Intestinal Mucosa. Frontiers in Immunology, 11(November). https://doi.org/10.3389/fimmu.2020.567801 DOI: https://doi.org/10.3389/fimmu.2020.567801
De Vuyst, L., & Neysens, P. (2005). The sourdough microflora: Biodiversity and metabolic interactions. Trends in Food Science and Technology, 16(1–3), 43–56. https://doi.org/10.1016/j.tifs.2004.02.012 DOI: https://doi.org/10.1016/j.tifs.2004.02.012
Di Cagno, R., Rizzello, C. G., De Angelis, M., Cassone, A., Giuliani, G., Benedusi, A., … Gobbetti, M. (2008). Use of selected sourdough strains of Lactobacillus for removing gluten and enhancing the nutritional properties of gluten-free bread. Journal of Food Protection, 71(7), 1491–1495. https://doi.org/10.4315/0362-028X-71.7.1491 DOI: https://doi.org/10.4315/0362-028X-71.7.1491
Gobbetti, M., De Angelis, M., Corsetti, A., & Di Cagno, R. (2005). Biochemistry and physiology of sourdough lactic acid bacteria. Trends in Food Science and Technology, 16(1–3), 57–69. https://doi.org/10.1016/j.tifs.2004.02.013 DOI: https://doi.org/10.1016/j.tifs.2004.02.013
Gobbetti, Marco, De Angelis, M., Di Cagno, R., Calasso, M., Archetti, G., & Rizzello, C. G. (2019). Novel insights on the functional/nutritional features of the sourdough fermentation. International Journal of Food Microbiology, 302(April), 103–113. https://doi.org/10.1016/j.ijfoodmicro.2018.05.018 DOI: https://doi.org/10.1016/j.ijfoodmicro.2018.05.018
Gobbetti, Marco, Minervini, F., Pontonio, E., Di Cagno, R., De Angelis, M., Minervini, F., … De Angelis, M. (2016). Drivers for the establishment and composition of the sourdough lactic acid bacteria biota. International Journal of Food Microbiology, 1–49. https://doi.org/https://doi.org/10.1016/j.ijfoodmicro.2016.05.022 DOI: https://doi.org/10.1016/j.ijfoodmicro.2016.05.022
Hammes, W. P., Brandt, M. J., Francis, K. L., Rosenheim, J., Seitter, M. F. H., & Vogelmann, S. A. (2005). Microbial ecology of cereal fermentations. Trends in Food Science and Technology, 16(1–3), 4–11. https://doi.org/10.1016/j.tifs.2004.02.010 DOI: https://doi.org/10.1016/j.tifs.2004.02.010
Koc, F., Arendt, E., Coffey, A., Ross, R. P., & Stanton, C. (2024). Impact of low FODMAP sourdough bread on gut microbiota using an in vitro colonic fermentation model. Frontiers in Microbiology, 15(November), 1–13. https://doi.org/10.3389/fmicb.2024.1496022 DOI: https://doi.org/10.3389/fmicb.2024.1496022
Kurtzman, C. (2011). The Yeasts, a Taxonomic Study (J. Fell & T. Boekhout, eds.). London: Elsevier Inc.
Landis, E. A., Oliverio, A. M., McKenney, E. A., Nichols, L. M., Kfoury, N., Biango-Daniels, M., … Wolfe, B. E. (2021). The diversity and function of sourdough starter microbiomes. ELife, 10, 1–24. https://doi.org/10.7554/eLife.61644 DOI: https://doi.org/10.7554/eLife.61644
McKenney, E. A., Nichols, L. M., Alvarado, S., Hardy, S., Kemp, K., Polmanteer, R., … Dunn, R. R. (2023). Sourdough starters exhibit similar succession patterns but develop flour-specific climax communities. PeerJ, 11, 1–24. https://doi.org/10.7717/peerj.16163 DOI: https://doi.org/10.7717/peerj.16163
Minervini, F., Dinardo, F. R., Celano, G., De Angelis, M., & Gobbetti, M. (2018). Lactic acid bacterium population dynamics in artisan sourdoughs over one year of daily propagations is mainly driven by flour microbiota and nutrients. Frontiers in Microbiology, 9(AUG), 1–15. https://doi.org/10.3389/fmicb.2018.01984 DOI: https://doi.org/10.3389/fmicb.2018.01984
Moroni, A. V., Dal Bello, F., & Arendt, E. K. (2009). Sourdough in gluten-free bread-making: An ancient technology to solve a novel issue? Food Microbiology, 26(7), 676–684. https://doi.org/10.1016/j.fm.2009.07.001 DOI: https://doi.org/10.1016/j.fm.2009.07.001
Mou, T., Xu, R., Li, Q., Li, J., Liu, S., Ao, X., … Liu, A. (2025). Screening of Antifungal Lactic Acid Bacteria and Their Impact on the Quality and Shelf Life of Rye Bran Sourdough Bread. Foods, 14(7), 1–12. https://doi.org/10.3390/foods14071253 DOI: https://doi.org/10.3390/foods14071253
Murniece, R., Reidzane, S., Radenkovs, V., Straumite, E., Keke, A., Kobrin, E., & Klava, D. (2025). Scald Fermentation Time as a Factor Determining the Nutritional and Sensory Quality of Rye Bread. Foods, 1–20. https://doi.org/https://doi.org/10.3390/foods14060979 DOI: https://doi.org/10.3390/foods14060979
Ramos, L., Alonso-Hernando, A., Martínez-Castro, M., Morán-Pérez, J. A., Cabrero-Lobato, P., Pascual-Maté, A., … Mujico, J. R. (2021). Sourdough biotechnology applied to gluten-free baked goods: Rescuing the tradition. In Foods (Vol. 10). https://doi.org/10.3390/foods10071498 DOI: https://doi.org/10.3390/foods10071498
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2025 Robert Leon-Suarez

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes have been made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.


